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RecombinantHumanACE2/ACEHProtein,hFcTag性能參數(shù),分子別名(Synonyms)ACE2;ACEH;ACE-2表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)HumanACE2/ACEHProteinisexpressedfromHEK293withhFctagattheC-Terminus.ItcontainsGln18-Ser740.[Accession|Q9BYF1-1]分子量大小(MolecularWeight)TheproteinhasapredictedMWof109.2kDa.Duetoglycosylation,theproteinmigratesto115-130kDabasedonSDS-PAGEresult.Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.活性(Activity)ELISAData:ImmobilizedSARS-COV-2SpikeS(B.1.1.529/Omicron)Trimer,HisTagat1μg/ml(100μl/well)ontheplate.DoseresponsecurveforHumanACE2,hFcTagwiththeEC50of13.7ng/mldeterminedbyELISA.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構(gòu)方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.C5a通過與髓源性抑制細(xì)胞 (MDSCs) 膜上的受體C5aR1結(jié)合,招募MDSCs至炎癥局部,抑制CD8+ T細(xì)胞增殖與功能。Recombinant Biotinylated Human IL-21 Protein,His-Avi Tag
糖苷內(nèi)切酶H是一種重組糖苷酶,能夠?qū)-糖蛋白中的高甘露糖和某些雜合型寡聚糖的殼二糖結(jié)構(gòu)進(jìn)行切割,去除糖蛋白中的N-連接高甘露糖。糖苷內(nèi)切酶H克隆自褶皺鏈霉菌(Streptomycesplicatus)。并在酵母中重組表達(dá)。本產(chǎn)品帶his標(biāo)簽,常應(yīng)用于抗體及其相關(guān)蛋白完全去糖基化。另外,我司還提供其他類型的糖苷酶,包括糖苷內(nèi)切酶S(Cat#20413ES),酵母重組表達(dá)的N-糖苷酶F(比活性:750000U/mL),酵母重組表達(dá)的N-糖苷酶F(比活性:100000U/mL)。儲(chǔ)存條件-15~-25℃保存,有效期1年。使用說明變性條件下蛋白質(zhì)去糖基化1)在水中加入1μLBuffer1和目標(biāo)糖蛋白(1-20μg),至終體積10μL;2)100℃溫度下煮沸10min使其變性,冰上冷卻,離心10秒;3)加入2μL的Buffer2,8μL去離子水,總反應(yīng)體積20μL;4)加入1-2μL的EndoH,輕輕混勻。在37℃孵育1-3h。5)65℃下熱失活10分鐘。非變性條件下蛋白質(zhì)去糖基化1)在水中加入2μL的Buffer2和目標(biāo)糖蛋白(1-20μg)至終體積為20μL。2)加入2~5μL的EndoH,輕輕混勻。3)37°C孵育4-24h。注意:在變性條件下大多數(shù)底物能夠更好的去糖基化,在非變性條件下可能需要增加EndoH的量和延長(zhǎng)孵育時(shí)間。Recombinant Mouse AMIGO2 Protein,His Tag高親和力和選擇性A2AR抑制劑的開發(fā):研究者正在開發(fā)新型的A2AR小分子拮抗劑,以提高藥物的療效和選擇性。
IdeSProtease全稱免疫球蛋白G降解酶(ImmunoglubulinG-degradingenzymeofStreptococcuspyogenes,IdeS),是由人類致病菌釀膿鏈球菌(Streptococcuspyogenes)產(chǎn)生并分泌至胞外的一種半胱氨酸水解酶。該蛋白酶具有極高的底物特異性,能識(shí)別IgG,在抗體下鉸鏈區(qū)的特定位點(diǎn)進(jìn)行酶切,使IgG水解為完整的F(ab’)2片段和Fc片段。IdeS可識(shí)別人源和其他多種動(dòng)物來源的IgG,比如人、兔、猴、綿羊以及人動(dòng)物嵌合IgG等。IdeS具有獨(dú)特的底物選擇性,可以作為工具酶應(yīng)用于抗體類藥物或抗體融合蛋白藥物的結(jié)構(gòu)表征分析。本產(chǎn)品利用大腸桿菌重組表達(dá),純度高,具有良好的酶切活性,是用于表征抗體、Fc融合蛋白和抗體-藥物復(fù)合物的有價(jià)值的工具。產(chǎn)品信息規(guī)格2000U/5000U產(chǎn)品性質(zhì)中文別名(Chinesesynonym)免疫球蛋白G降解酶英文別名(Englishsynonym)IdeSProtease來源(Source)大腸桿菌表達(dá)標(biāo)簽(label)N-terminalHisTag純度(Purity)經(jīng)SDS-PAGE及HPLC分析,純度>95%分子量(Molecularweight)35.3kDa緩沖液組分(Buffer)50mM磷酸鈉,150mMNaCl(pH6.6),50%glycerol
RecombinantBiotinylatedHumanOX40/TNFRSF4/CD134Protein(PrimaryAmineLabeling),hFcTag性能參數(shù)分子別名(Synonyms)OX40Lreceptor;CD134;TNFRSF4;Ly-70表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)BiotinylatedHumanOX40/TNFRSF4/CD134Protein(PrimaryAmineLabeling)isexpressedfromHEK293withhFctagattheC-Terminus.ItcontainsLeu29-Ala216.[Accession|P43489]分子量大小(MolecularWeight)TheproteinhasapredictedMWof46.8kDa.Duetoglycosylation,theproteinmigratesto72-75kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGE制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).儲(chǔ)存條件Theproductshouldbestoredat-85~-65℃for1yearfromdateofreceipt.Recommendtoaliquottheproteinintosmallerquantitieswhenfirstusedandavoidrepeatedfreeze-thawcycles.在生物制藥工業(yè)中,腸激酶用于生產(chǎn)重組蛋白質(zhì)藥物,通過專一性切割去除不需要的序列,提高藥物純度和活性。
RecombinantBiotinylatedHumanHLA-A*02:01&B2M&MAGE-A4orMAGE-A8(KVLEHVVRV)MonomerProtein,His-AviTag性能參數(shù)分子別名(Synonyms)MAGE-A4orMAGE-A8;MAGE-A4;MAGE-A8;表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)BiotinylatedHumanHLA-A*02:01&B2M&MAGE-A4orMAGE-A8(KVLEHVVRV)MonomerProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.ItcontainsGly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)andKVLEHVVRVpeptide.[Accession|A0A140T913(HLA-A*02:01)&P61769(B2M)&KVLEHVVRV]分子量大小(MolecularWeight)TheproteinhasapredictedMWof50.50kDa.Duetoglycosylation,theproteinmigratesto52-62kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).儲(chǔ)存條件Theproductshouldbestoredat-85~-65℃for1yearfromdateofreceipt.透明質(zhì)酸及其衍生物由于其生物相容性和生物降解性,被用作藥物的控釋載體。Recombinant Cynomolgus NGAL/Lipocalin-2 Protein,His Tag
CB1受體分布于大腦和外周神經(jīng)系統(tǒng)中,與多種生理功能有關(guān),包括疼痛感知、食欲調(diào)節(jié)、情緒調(diào)節(jié)。Recombinant Biotinylated Human IL-21 Protein,His-Avi Tag
RecombinantBiotinylatedHumanHLA-A*03:01&B2M&KRASG12V(VVGAVGVGK)MonomerProtein,His-AviTag性能參數(shù)分子別名(Synonyms)MHC;KRAS;K-Ras2;KRAS2;C-K-RAS;CFC2;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KRAS1;KRAS2;NS;NS3;RASK2;GTPaseKras;KI-RAS;RALD表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)BiotinylatedHumanHLA-A*03:01&B2M&KRASG12V(VVGAVGVGK)MonomerProteinisexpressedfromHEK293withHistagandAvitagattheC-TerminusItcontainsGly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M)andVVGAVGVGKpeptide.[Accession|NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVGAVGVGK]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof50.09kDa.Duetoglycosylation,theproteinmigratesto51-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).Recombinant Biotinylated Human IL-21 Protein,His-Avi Tag